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CHM.212631 - Proclin 300 Alt. -preservative for use in IVD manufacturing
ProClin 300 is a widely used preservative in molecular biology and laboratory settings, known for its effectiveness in preventing microbial contamination in aqueous solutions. It is a broad-spectrum biocide that contains a mixture of isothiazolinones, primarily 5-chloro-2-methyl-4-isothiazolin-3-one (CMIT) and 2-methyl-4-isothiazolin-3-one (MIT). Below is a detailed overview of the usage of ProClin 300 in various applications:
1. Preservation of Reagents and Buffers
ProClin 300 is commonly added to aqueous reagents, buffers, and solutions to inhibit the growth of bacteria, fungi, and algae. Its effectiveness at low concentrations (typically 0.02–0.1%) makes it ideal for preserving:
  • Molecular Biology Buffers: Such as Tris-buffered saline (TBS), phosphate-buffered saline (PBS), and hybridization buffers.
  • Enzyme Solutions: Including restriction enzymes, polymerases, and ligases.
  • Nucleic Acid Solutions: Such as DNA and RNA storage buffers.
By preventing microbial contamination, ProClin 300 ensures the stability and longevity of these reagents, reducing the risk of experimental errors.
2. Cell Culture Media and Supplements
In cell culture applications, ProClin 300 is used to preserve media, sera, and supplements that are prone to microbial growth. It is particularly useful for:
  • Long-term Storage: Preventing contamination in media and supplements stored at 4°C or room temperature.
  • Reducing Antibiotic Use: ProClin 300 can partially replace antibiotics in cell culture media, minimizing the risk of antibiotic resistance.
However, it is important to note that ProClin 300 is cytotoxic at high concentrations and should not be used in media intended for direct cell culture.
3. Diagnostic Kits and Assays
ProClin 300 is widely used in diagnostic kits and assays to preserve the integrity of reagents and ensure consistent performance. Examples include:
  • ELISA Reagents: Such as coating buffers, wash buffers, and enzyme conjugates.
  • Lateral Flow Assays: Preserving antibodies and other biomolecules used in test strips.
  • Clinical Chemistry Reagents: Ensuring the stability of calibrators and controls.
Its compatibility with proteins and antibodies makes it a preferred preservative in immunoassays.
4. Protein and Antibody Stabilization
ProClin 300 is often added to protein and antibody solutions to prevent microbial degradation. It is particularly useful for:
  • Monoclonal and Polyclonal Antibodies: Preserving stock solutions and working dilutions.
  • Enzyme Solutions: Such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) used in immunoassays.
Its low interference with protein function ensures that the biological activity of these molecules is maintained.
5. Molecular Biology Kits
Many commercial molecular biology kits, such as those for DNA/RNA extraction, PCR, and sequencing, contain ProClin 300 to extend shelf life and prevent contamination. It is especially useful in:
  • Master Mixes: Preserving PCR and qPCR reagents.
  • Nucleic Acid Purification Kits: Protecting lysis buffers and wash solutions.
6. Industrial and Research Applications
ProClin 300 is also used in industrial and research settings to preserve aqueous solutions, including:
  • Cosmetic and Personal Care Products: As a preservative in shampoos, lotions, and other water-based formulations.
  • Paints and Coatings: Preventing microbial growth in water-based products.
  • Water Treatment: Controlling microbial contamination in industrial water systems.
7. Advantages of ProClin 300
  • Broad-Spectrum Activity: Effective against bacteria, fungi, and algae.
  • Low Concentration: Requires only 0.02–0.1% for preservation.
  • Compatibility: Works well with proteins, antibodies, and nucleic acids.
  • Stability: Remains effective over a wide pH range (2–9) and at elevated temperatures.
8. Safety and Handling
  • Toxicity: ProClin 300 is toxic at high concentrations and should be handled with care. Avoid direct contact with skin and eyes.
  • Disposal: Dispose of according to local regulations for hazardous chemicals.
  • Storage: Store in a cool, dry place away from direct sunlight.
9. Limitations
  • Cytotoxicity: Not suitable for use in cell culture media or solutions intended for live cells.
  • Interference: May interfere with certain assays if used at high concentrations.

CHM.212631 - Proclin 300 Alt. -preservative for use in IVD manufacturing

SKU: CHM.212631
€35.00Price
Quantity
Will ship 7-14 days from Malta

  • 1. Chemical Composition

    • Active Ingredients:

      • 5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT): ~2.15% (w/w).

      • 2-Methyl-4-isothiazolin-3-one (MIT): ~0.35% (w/w).

    • Inert Ingredients: The remaining composition includes water and magnesium salts (e.g., magnesium nitrate and magnesium chloride) as stabilizers.

    2. Molecular Structure

    • CMIT:

      • Chemical Formula: C₄H₄ClNOS.

      • Molecular Weight: 149.6 g/mol.

      • Structure: Contains a chloro-substituted isothiazolinone ring.

    • MIT:

      • Chemical Formula: C₄H₅NOS.

      • Molecular Weight: 115.15 g/mol.

      • Structure: Contains a methyl-substituted isothiazolinone ring.

    3. Physical Properties

    • Appearance: Clear, pale yellow to amber liquid.

    • Odor: Slight characteristic odor.

    • Density: ~1.02–1.05 g/cm³ at 25°C.

    • Solubility: Miscible with water and water-miscible solvents (e.g., ethanol, methanol). Insoluble in non-polar solvents like hexane.

    • pH: Typically between 2.0 and 5.0 in aqueous solutions.

    4. Chemical Stability

    • Thermal Stability: Stable at room temperature but may degrade at elevated temperatures (>40°C) or under prolonged exposure to heat.

    • pH Stability: Effective over a wide pH range (2–9), making it suitable for use in acidic, neutral, and slightly alkaline solutions.

    • Light Sensitivity: Stable under normal lighting conditions but may degrade under prolonged exposure to UV light.

    5. Reactivity

    • Compatibility: Compatible with most aqueous solutions, proteins, and nucleic acids. However, it may react with strong reducing agents, oxidizing agents, or nucleophiles (e.g., thiols and amines).

    • Decomposition: May decompose under extreme conditions, releasing small amounts of chlorine gas or other degradation products.

    6. Antimicrobial Activity

    • Mechanism of Action: ProClin 300 disrupts microbial cell membranes and inhibits metabolic enzymes, leading to cell death.

    • Spectrum of Activity: Effective against a broad range of microorganisms, including:

      • Bacteria: Gram-positive and Gram-negative bacteria.

      • Fungi: Yeasts and molds.

      • Algae: Prevents algal growth in aqueous solutions.

    7. Concentration and Efficacy

    • Effective Concentration: Typically used at concentrations of 0.02–0.1% (v/v) in aqueous solutions.

    • Minimum Inhibitory Concentration (MIC): Varies depending on the microorganism but is generally in the range of 1–10 ppm for bacteria and fungi.

    8. Safety and Toxicity

    • Toxicity: Toxic at high concentrations. CMIT and MIT are skin and eye irritants and may cause allergic reactions in sensitive individuals.

    • Environmental Impact: Toxic to aquatic life at high concentrations. Proper disposal is required to minimize environmental impact.

    • Handling: Use personal protective equipment (PPE) such as gloves and safety goggles when handling ProClin 300.

    9. Storage and Shelf Life

    • Storage Conditions: Store in a cool, dry place (15–25°C) in tightly sealed containers away from direct sunlight.

    • Shelf Life: Typically 1–2 years when stored under recommended conditions.

    10. Analytical Methods

    The concentration and stability of ProClin 300 can be assessed using:

    • High-Performance Liquid Chromatography (HPLC): To quantify CMIT and MIT levels.

    • Gas Chromatography-Mass Spectrometry (GC-MS): For detecting degradation products.

    • Microbial Challenge Tests: To evaluate antimicrobial efficacy.

    11. Applications

    ProClin 300 is used in a wide range of applications, including:

    • Molecular Biology: Preservation of buffers, enzymes, and nucleic acid solutions.

    • Diagnostics: Stabilization of reagents in immunoassays and clinical chemistry.

    • Industrial: Preservation of water-based products such as paints, coatings, and cosmetics.

    • Pharmaceuticals: Protection of aqueous formulations from microbial contamination.

    12. Regulatory Compliance

    ProClin 300 complies with various regulatory standards, including:

    • EPA (Environmental Protection Agency): Approved for use in industrial applications.

    • EU Biocidal Products Regulation (BPR): Compliant with European Union regulations for biocidal products.

    • FDA (Food and Drug Administration): Approved for use in certain diagnostic and pharmaceutical applications.

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